English translation for "embryo fibroblast"
|
- 胚胎成纤维细胞
Related Translations:
- Example Sentences:
| 1. | Culture of the attenuated a66 strain of duck hepatitis virus in chicken embryo fibroblast
66弱毒株在鸡胚成纤维细胞上的培养 | | 2. | Development of chicken embryo fibroblast cell line resistant to j subgroup avian leukosis virus infection
亚群禽白血病病毒的鸡胚成纤维细胞系建立 | | 3. | Construction of recombinant fowlpox virus coexpressing aiv ha and ndv f . for the construction of transfer vector pfgs11haf , aiv ha gene of f strain in puc18ha and ndv f gene of f48e8 strain in puc19f were removed and inserted into pfgs11 . recombinant fowlpox viruses ( rfpv ) coexpressing aiv ha gene and ndv f gene were constructed by using different promoters of ps and pe / l . recombinant rfpvs were derived by dosper liposome - mediated transfection with the two transfer vectors on chicken embryo fibroblast ( cef ) monolayer cultures which were infected by wild type fpv chinese vaccine strain 282e4 3 - 4 hours earlier
Puc18ha和sk质粒同时经hind 、 kpn酶切后连接得中间质粒skha ；将质粒skha用bamhi酶切回收ha基因插入到插入载体pfgs11中的bamhi位点，通过酶切鉴定获得了pfgs11ha ；将含ndvf基因的质粒puc19f用hind 、 sal酶切经klenow酶补平插入到经sma酶切后的skifn中pe / l启动子下获得中间质粒skf ，再将质粒skf和puc18质粒先分别用ecor 、 xho酶切klenow补平，后再共同用sac酶切连接得puc18pelf ， sal酶切回收pe / l - f基因盒插入到pfgs11ha的sal位点，通过酶切鉴定获得了pe / l - f与ps - ha同向的表达载体pfgs11haf 。 | | 4. | Meq protein , highly expressed in the insect cell line sf9 by the baculovirus vector was immunized into balb / c mice and the immunized spleen cells were collected and fused with the tumor cell line sp2 / 0 via peg - 1000 in vitro . the hybridoma cells were cloned and screened for the ability of anti - meq mcab secretion by fa with the mdv ga infected chicken embryo fibroblast ( cef )
利用通过杆状病毒载体在昆虫细胞系sfg上高度表达的meq蛋白产物免疫balb / c小鼠，然后收获其免疫脾细胞并与肿瘤细胞系spz / 0通过peg于体外融合;获得的杂交瘤细胞被克隆并通过与mdv感染的鸡胚成纤维细胞( cef )做免疫荧光试验( fa ) ，进行其分泌抗meq单克隆抗体( mcab )能力的筛选。 | | 5. | From dead chicken which infected infectious stunting syndrom of our province , one virue was isolated using spfeggs , chicken embryo fibroblast , mdck18 , and vero cell . this virus was unable to agglutinate chicken erythrocytes . in order to definite the pathogeny of infectious stunting syndrom . physical and chemical specific property , types of the nucleic acid of the isolated virus , recurrent infection and other biological property determination and indirect elisa test proved it as a parvoviruses like strain of chicken
为确定该病的病原，对所分离病毒进行了理化特性测定、病毒核酸型别测定、动物回归试验等生物学特性测定，证明该分离病毒与细小病毒科( parvovirdae )细小病毒属( parvovirus )的鸡细小病毒( chickenparvovirus )特性基本相符，核酸型为dna型。 | | 6. | From dead chickens , one virrus was isolated by using eggs and chicken embryo fibroblast . lt was able to agglutinate chicken ' s erythrocytes and this heamagglutination could be inhibited by newcastle disease antiserum . this strain ' s biological property was tested by barren spot , cross - enutralization and cross - heamagglution inhibited and it was found that it was homological with the standard newcastle disease virus ( ndv ) virulent strain and avirulent strain but it had some diference with the standard strain
本实验采用spf鸡胚及鸡胚原代成纤维细胞，从河北省某鸡场新城疫免疫抗体很高的病死鸡的脑组织中分离得到一株病毒。此株病毒能凝集鸡的红细胞，并且这种凝集可以被特异性抗血清所抑制。 |
- Similar Words：
- "embryo dormancy" English translation, "embryo engineering" English translation, "embryo esker" English translation, "embryo extract" English translation, "embryo extract apparatus" English translation, "embryo fisheries" English translation, "embryo fusion" English translation, "embryo grafting" English translation, "embryo growth development factor" English translation, "embryo growth retardation" English translation
|
|
|
